Hi everyone, I’m new here and also in the field of microscopy.
My research investigates the interactions between minerals and fatty acids vesicles, and to do so I’m using a microfluidic system.
As I’m working with iron sulfide, I’m conducting experiments in an anaerobic glove box. Because of budget constraints and mostly the limited space in the glove box, I’m planning to build an openflexure microscope that I can put inside.
Before starting working on this, I was looking for some advice as it’ll be my very first time doing something like this, I only have experience in 3D printing.
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is it possible to control the stage remotely? Like adding a bluetooth component so I can plug in the microscope with the electricity inside the glove box. In this way I won’t have to make a hole in the box to attach it to the outside computer.
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With the microscope, I’ll need to look at both micron and nanometers structures (usually the vesicles size range is between 400 nm and 15 micrometer). Would the Raspberry pi camera be able to visualise those small versicles or would be better to consider another camera? And can the Raspberry pi camera take slow motion videos?
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I’ll need to use fluorescence and pseudo-phase as it’ll be easier to visualise the mineral. Would you recommend to build the Delta Stage microscope or customise the high resolution microscope (which maybe are the same? I got a bit confused on this)
In the future, once I’ll build the first one and have a better understanding on how to do it and how it works, I plan to further customise it to do microfluidic imaging/video analysis.
Thank you!
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