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About v6 reflection illumination microscope

Hi all, I’m trying to build a v6 reflection illumination microscope. The ‘reflection illuminator.stl’ file includes three parts, but I can’t work out how to use one of the circular parts with a hole...

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Newbie seeking advice on using OFM for scanning diatom slides

Hello everyone, I’m working with diatoms and was looking into possible solutions for improving the standard methodolgy via virtual slides. Commercial slide scanners are very expensive and I’ve...

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How to use the nano converter plate and gripper? - Sangaboard alternative

Hi, I’m using the Sangaboard workaround using the Arduino Nano alternative. I printed the nano_converter_plate-pi4.stl and nano_converter_plate_gripper.stl, but I’m not sure how to use them to...

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Camera Disconnected

Hi all, logged into the web interface for first boot up and was met with a message saying the camera was disconnected. I have checked to see that I have plugged the camera into the correct slot and I...

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Resources for learning

Hi all! I’m a Statistician and I’m interested in participating in this community. I’ve bought the IORodio kit and will be assembling the v7 microscope in a few weeks, but I’m particularly interested...

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Upgrading Pi and camera module for delta stage

Hello, I am currently building using a delta stage in my setup and was wondering if anyone knows whether it is possible to upgrade to the pi5 and camera module v3 (instead of the reccomended pi 4 and...

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Microscope's pink screen

Hello,I’ve completed the assembly and successfully connected my microscope to a screen, but the image appears to have a pinkish tone(the image will be attached below). I’ve already connected it to...

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Unable to focus

Hi i am new here. i am using standard hi resolution microscope but i am unable to focus on the slide. i am using standard hi resolution microscope with labomed acromatic lens with 40x / 0.65 160...

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Disconnecting of network during scanning

Hi everyone, I have been trying to do a scan on a malaria positive blood sample, but after some two or more points of navigation or scanning the system automatically disconnects and this is the first...

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Delta stage increasing gear ratio

Hello. I am currently using the delta stage in a setup which involves scanning the sample. However due to the very small step sizes, the 28BYJ-48 motors struggle and some images have lateral movement...

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Need help adapting OFM software to a cheap microscope with motorized z axis

Hi all question about a cheap microscope project I’m planning and whether OpenFlexure software can help. What I have / plan Microscope: cheap model only coarse focus (no fine focus knob):...

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Broke the X axis of the stage due to over rotation

Hi, i assembled my high resolution microscope and was testing it out on the sample, when i put the X value to be very high, causing the motors to keep rotating and suddenly heard cracking sounds,...

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Diffuse Led Light with paper: does it have meaning in this way?

Hi everyone! First post here. I’m building the high resolution microscope v7. Ok, here’s the thing: I bought a bunch of lenses for the illumination (here are the lenses I have:...

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Piezo nanoactuator w/o a flexture

This might be a rather silly question, I apologize to everyone. I have a very small sample about 5x5x1 mm (a flat disk) which I would like to move by 0-5 micron along one axis under my microscope...

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Unable to focus using x60 or x100 lens

Dear all, First of all, thanks a lot for this great project. I was looking into it and start building it for a private school in Belgium. And that for two reasons: to enhance kids experience by...

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Trouble connecting to the server

I keep getting connection errors when trying to access my project files. Has anyone else run into this? What steps actually helped fix it? 1 post - 1 participant Read full topic

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Stack Scan not working

I have a couple of challenges with my OFM setup. I have modified it for malaria detection using two polarizers. The OFM often shuts off when I use the stack scan, and the camera-stage calibration does...

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Color Calibration and Magnification issues

Hi, i have assembled my high res microscope with a 40x objective and have started using it after all calibrations. This is the image from my microscope (ignore the black lines they are most probably...

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Modifying OpenFlexure: Where to start?

Hello everyone, I am an electron microscopist, and I discovered OpenFlexure project some months ago. I was amazed! I quickly bought the necessary components, and built my first microscope. It was...

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Clean aperture for coherent illumination

Hello everyone, I’m working on building an OpenFlexure microscope with coherent illumination using a cheap laser module. I disassembled and removed the built-in lens at the tip of the laser module...

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Microscope stoped working

I took the microscope apart to clean the camera sensor. when I reassembled it I could no longer connect remotely. I connected monitor, mouse and keyboard. The raspberry pi boots up but when I run...

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New build cannot focus or calibrate

Hello I recently built the v7 high resolution microscope as a gift for my son, I bought the Achromatic lens and board from iorodeo along with other parts. I tried using the “plan ASC 40/0.65 160/0.17...

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Title: Circular artifacts/rings appearing in Raspberry Pi Camera microscope...

Hi everyone, I’m using a Raspberry Pi 4 with a Pi Camera.I started seeing strange circular artifacts/ring patterns that were not present before. I captured two images for comparison, and both were...

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Which SD card to choose?

Hi all, I am still not sure this is the only root for the issue I am experiencing but with the great help from this forum it seems that I am getting “Network Error” message on Server v3 during the...

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No Stage Connected

Hi, looking for some help. I’ve read a few older threads with similar problems, but I’m guessing some people fixed it without following up. I get “No stage connected” I’ve got a pi4 with Sangaboard...

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