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Help with translating the server and a mass distribution project

Hi folks, We at GSIO Georgia, have just finished building the low cost basic optics module v6 microscope and have been testing it extensively with great results ( see pictures below). We are a small...

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Is my old hardware good enough?

Sorry, I don’t know where to start after seeing your booth at FOSDEM. I couldn’t find a definitive list of recommended parts. I wonder if my old camera v2.1 module here is good to start with...

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Methods for Determining Minimum Resolution

Hi, given that larger resolution images need more processing power to work with and take up more storage space, it is preferable to have lower resolution images if possible. I was wondering if anybody...

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What is the vertical travel of the lever per step?

Hi! So my esp32-based controller box for the Delta Stage is almost 100% complete. I have added end stop switches into the feet and they work really well. However, since the project is for 2P...

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Delta stage accumulated error

Hello, first of all thanks for this amazing project. I’m impressed by what has been achieved here. I have just finished putting together a delta stage microscope using the latest version of...

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No open serial port found on delta stage with Sangaboard V0.2

Hi everyone, I have built an OpenFlexure Delta Stage microscope with a Sangaboard V0.2. For the Sangaboard V0.2, I ordered the PCB online and then soldered the circuit components onto the PCB myself....

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Intermittent Black Screen on OpenFlexure Live View Seeking Troubleshooting Tips

Hi Everyone, Iam experiencing an intermittent black screen issue in the live view of my OpenFlexure microscope. The video feed occasionally goes completely blank, even though the microscope itself...

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Leg struts fail to print

Experienced 3D printer here, first time trying to print the OpenFlexure on our Ultimaker S5 FDM 3D Printer using PLA. As I hope you can see from the attached image, the horizontal struts that span the...

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How to home the motors?

Hello, there is something I do not quite understand abou the OpenFlexure. ■ How are the motors homed?, and ■ How to proceed if, e.g. while first installing and getting familiar with it, the focus is...

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Initial focus/calibrate problems after build completion

I have a number of issues post-build trying to focus on my first test slide. I’m listing them in case they are related to each other. I cannot get the slide to focus. What I see on the screen is...

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I've snapped twenty o-rings

I just snapped my twentieth o-ring trying to install them. I’ve been successful ~4 times. I’m using the recommended o-rings straight from McMaster-Carr. I put a lot of force on the large gear, pushing...

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Building a microscope for meteorite viewing

Hi, I’m interested in building either the normal microscope or the delta stage to put in a museum (behind glass) and ‘fly’ over a meteorite sample. Later ideas might include letting the visitors...

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A desperate newbie can't get the camera to work

Hi all, a newbie here, I yet have very little experience with raspberry pi and electronics in general. Context: I REALLY want to get openflexure to work in order to take images of soil samples. I have...

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500 deque mutated during iteration error

I’m encountering a “500 deque mutated during iteration” error while using the OpenFlexure Microscope software. This issue appears when attempting to use the live preview or other functionalities....

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Recurring Low-Cost Camera Problems

We are two college students building the low-cost microscrope, and we keep running into the same few problems. We are unsure if they are related, and would appreciate any help: We keep getting a...

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Spinning Z axis Gear

I’ve built the Version 7 microscope. The basic electronics work and I get illumination and an image on the screen, but I have a couple of problems. 1) I used cap headed bolts for the large gears and...

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4th motor integration on Sangaboard v0.5

Hi, First of all, thanks for this great device and community! I would like to implement structured illumination on an OFM v7. For this, I want to intergrate a fourth motor (28BYJ-48) on a Sangaboard...

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Dotstar grid illumination issues!

Hello! I’m very happy to report that I’ve printed my delta stage microscope, have assembled it along with assembling an adafruit dotstar LED 8x8. I’ve followed the instructions as per the “LED grid...

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Why is there distortion in my microfluidic droplet images?

Hi everyone, I am currently working on microfluidic droplet generation experiments. When I capture images of the droplets under the microscope, I notice that the images appear distorted (for example:...

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Working with sangaboard hardware/library

hey guys, hope you’re all doing well. I’ve been tasked with making the project compatible with a raspberry 5 and so far it has been rough due to my lack of understanding of the inner workings of the...

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Optics module choice

Can someone explain to me what the reflection optics module does? I keep seeing epi-fluorescence being associated with it but are they equivalent? For example if i want to see the surface of a...

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Slide positioning

Is the openflexure designed so that the slides are upside down (coverslip downward)? If yes how do you keep the coverslip from falling? Some samples that im interested in are blood, hair, onion skin...

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My lens is too high

Hello dear openFlexure community, I’ve just finished assembling my microscope. However, I’m facing an issue where my objective lens is sticking out too far at the top, and I’m wondering what I might...

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Stripped thread

The thread for my sample clip on the delta stage is too loose. I’m not sure if it was a print defect or me stripping it by accident. Is there anything I could do to fix it except spending another...

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Need help compiling STL

I wanna modify the optics module but I don’t know scad. I’m hoping that someone can help me compile an stl of the optics module for reflection delta stage without the mounting part (I think it’s the...

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