I have a number of issues post-build trying to focus on my first test slide. I’m listing them in case they are related to each other.
- I cannot get the slide to focus. What I see on the screen is totally out of focus, but the color is white, then light green when I insert the slide, so I know the slide object (honey bee part) is centered. If I disconnect the Z stepper motor and move through the entire gear range, it never comes even remotely into focus. I do see a moment of focus on what appears to be perhaps dust on glass, and if I remove the slide the same dots appear, background is now white, so I think I am focusing on the objective lens glass itself, not the slide object.
- When I try auto-calibrate, I hear the stepper motors, initially short buzz bursts, eventually longer bursts, but the gears do not turn, at least that I can see. I removed X and Z motors, the gears seem to move freely by hand. The X motor is quite hot to the touch, Z is slightly warm, Y is cold.
I bought all my parts from IO Rodeo, including the objective, Sangaboard and PI 4B, the motors are 5V, so I assume the parts are correct. One issue I did have during build was that the objective extended up onto the slide deck, so I couldn’t get a slide into the clips. Rather than push the objective to the top of its slot like the instructions say, I had to lower it before tightening the screw such that the objective just clears the bottom of the slide.
Hoping for suggestions,
Thanks, Kevan
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