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Openflexure stage program in microscope

Hello, I’ve successfully built confocal microscope. Here is the picture of 1 euro star from this microscope: Now I’m trying to optimize my program to make it work with smaller steps (link to old...

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Nano Converter plate incompatible with RPi 3b

Hey! I was building the microscope. Just finished printing the electronics drawer for RPi 3 Sangaboard 4 (electronics_drawer-pi3_sangav0.4) and the nano_converter_plate (which I found from the docs,...

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Spontaneous disconnection of the delta stage

Hey everyone, I built a delta stage microscope. It is a really awesome device. However, after some time of usage the stage does not move anymore and when I reload the webpage It says “Stage...

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Microscope v7 stage is jumping / skipping / rewinding

I just finished assembling a v7 microscope and the assembly ultimately went fairly smoothly. Everything is mostly working, and I’m able to capture some really very nice images of some assorted sample...

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Which optics part for my old microscope objective?

HI, I’m not sure if i should print optics_picamera_2_rms_f50d13 or (beamspliiter version) for my objective. I’m building high resolution version with pi cam 2. The objective’s thread size is like...

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Simplified OpenFlexure microscope for visual inspection by children

Good evening, We’re having some fifty young school children coming over to visit our research center in two weeks time. We thought it could be insightful and empowering to let each one of them...

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Adjusting the condenser

Hi there, I just got my first OFM up and running and am deeply impressed, really brillant work! However, I still have one issue: Regarding adjusting the condenser position, in the assembly...

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Extending OpenFlexure to Schools

This is a follow up to a Zoom call with Richard Bowman regarding adaptation of the OpenFlexure microscope for use in schools. The build instructions call for use of the Sangaboard, which (a) does not...

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Focusing and Chromatic Aberration Issues

Hello everyone, I have constructed an OFM version 7, equipped with an Olympus PLN 100X Oil Immersion Objective. The attached image is sourced from cellular pathology. However, I am encountering...

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How do I connect Arduino to stepper motors and get code

Hi, I am new, I have built a Microscope. I have searched and been unable to find how to wire up my stepper motors to an arduino. Any pictures I see are so small I cant make anything out. Where can I...

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"Camera disconnected"

I just assembled my OFM v7 high resolution. Instead of an image, I just get “camera disconnected.” I looked through the 2020 thread about the issue and tried the solutions there, to no avail. I have...

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Microscope interface unreachable via browser, but I'm connected via SSH

I was trying a new camera, plugged in my microscope, and then tried to connect as per usual, but the web interface would not open. (Unable to connect, cant establish connection to server) I pinged the...

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Rotate display in server

I am making a bit of progress on the field dissection microscope. Based on the best way to run the ribbon cable the image on the screen is 180 degree rotated from the object in reality. Which seeing...

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Monochrome Camera Option

I’m trying to use the transmitted light version of the Delta stage to make a rig that measures blood vessel diameter. The problem is – I need a monochrome camera to make the diameter tracing software...

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Camera resolution

Dear OFM developers, I know this topic has come up before (e.g. https://openflexure.discourse.group/t/stream-resolution-options/713. However, it would be great if somebody could check if 1640x1232...

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Focusing problem

HEllo group: I built this new microscope and cannot figure out why 1/3 of the image is out of focus. I have tried with different objectives, different magnifications, reposition the lenses, and the...

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Sangaboard v0.5.3 not recognized/working

So I went around and around with this all day, looking at all the posts from @filip.ayazi et al, trying to figure out why this brand new board doesn’t seem to be recognized. It seems that the RPi is...

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Changing beamsplitter size? SCAD changes?

I finally got a beamsplitter plate to try out some reflection microscopy, but I’ve been struggling with changing the SCAD files to adapt to the size of my current beamsplitter (I don’t have a way to...

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Switching from low to high res

Hi, How easy is to switch from a low res using PI cam lens to objective? DOes it require the reprint of the main body part or just the additional parts? thank you 8 posts - 2 participants Read full...

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USB Web camera Lens and Camera Platforms

Hi all, I’m looking to modify my setup a little and use a usb webcam rather than the camera port and was wondering if anyone ever ended up making a lens spacer / camera platform that worked well with...

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