Any issues with PETG?
Hi, I’m looking to build my first OFM and recently got a lovely 2kg spool of very dark (almost black) recycled PETG prusament. I’d print with PLA as per the instructions but: really want to use...
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Help to downgrade Python version in Bookworm (Raspberry Pi OS)
Hi everyone I would like to request some help to downgrade the Python version in Bookworm (the latest version of Raspberry Pi OS). Disclaimer: This post may not be related directly to the OpenFlexure...
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Is the included lens tool correct?
My pi camera didn’t come with a lens removal tool. The printed tool included in the pack of STL files for version 7 & 6 both look nothing like the one that comes directly from pishop or other...
View ArticleIncrease Z travel range
Hi, for a specific application I would like to increase the Z travel range, to be able to focus on a microfluidic structure ~ 3 mm above the usual focal plane, i.e. move forth and back from the slide....
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ValueError: Stage response to 'p?'
Hi Instead of printing an entire Openflexure Microscope I printed gears to motorise an old Olympus EC. It works! At first, I struggled with the stage mapping, as the x- and y-axis have different gear...
View ArticleAlternative file options other than STL for block/delta stages?
Hi!! I’m currently using the openflexure delta stage as a starting point in my thesis but I want to improve on the design to fit my needs and I want to better understand how the mechanism works! I was...
View ArticleFluidNC Controlling Delta Stage
Hello Everyone, I recently built a Delta Stage and featured it in my latest YouTube video.DIY Reverse Engineering Tools I’ve really enjoyed using it under my AmScope microscope to view silicon wafers...
View ArticleSangaboard V0.4 only works with a usb cable
Hello, I am building a microscope with a raspberry pi 4 and a Sangaboard v0.4 soldered by myself, I make it work with the image on the openflexure page, but only with a usb cable that connects the...
View ArticlePMMA lens 5mm focal, 12.7mm diameter
I am having difficulties in getting the PMMA condenser lens. Can anyone please point me to a current supplier ? I am not sure why but aliexpress delivers only rubbish when I search for PMMA lens 5mm...
View Article4x Objective lens can't focus
Hello, I’m new to the openflexure microscope. Thanks to lots of people built up this system, I’ve made my system. Now I found that I cannot focus with my 4x objective lens(Olympus UPLFLN 4X...
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Wiring help with delta stage with Arduino?
Hi guys! I’m trying to wire up the motors with the arduino. The fitzing diagram says the xyz motors are connected with xyz actuators respectively but on the delta stage there doesn’t seem to be...
View ArticleNo SangaStage device was found during port scanning
Hi, New here… I finally got the SangaBoards v0.5.3 (the last piece I was waiting for) in and was very excited to get everything running today with my OFM v7. Unfortunately, I’ve run into a few...
View ArticleFinding overall magnification when using a tube lens and objective lens
Hi! I was using an actual microscope and realised that when understanding magnification, you have the objective lens and then the eyepiece lens which provide different magnifications so if the...
View ArticleInverted fluorescence microscope for cell culture
We are currently in need of assistance in identifying whether there exists a variant model of this inverted microscope that is compatible with cell culture plates and flasks. The distance between...
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Delta stage autofocus with x20 objective lens is not working/z-axis not...
Hi guys! We set up the delta stage accordingly and got a x20 objective lens but when we try to autofocus, nothing seems to happen. Before focusing: After focusing : From what it seems, the image has...
View ArticleAutomated image capture - delta stage
Hi, I am trying to do an image capture on the delta stage every 30 min for 24h. Is there a softwere compatible with delta stage able to do that or do I need to build one myself? Thanks for all the...
View ArticleOptics configuration for a 7.9 mm diagonal sensor
Hello! I’m quite new to digital microscopy and I can’t figure out how to incorperate a camera with a larger sensor into the openflexure microscope: I’ve been using the Arducam 12MP camera which uses...
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Sangaboard versions 0.3 and 0.5 difference
Hello everyone, So i have started to look into openflexure project 2 years ago and i bought all the necessary materials, which includes printing the sangaboard v0.3 and buying all the components for...
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We use 100x 1.25NA oil 160/0.17 wd0.14 objective lens but we require better...
we use 100x 1.25NA oil objective lens，but the pictures still look unclear. Can you please provide solutions to better improve the resolution for accurate detection? 2 posts - 2 participants Read full...
View ArticleStream Preview Disabled & 500 Error
We’ve recently come upon a new (to us) issue. We have a microscope that was previously working. We are connecting to it locally, but now when we click “Connect”, we get a message stating “Stream...
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