Why is there distortion in my microfluidic droplet images?

Hi everyone,

I am currently working on microfluidic droplet generation experiments. When I capture images of the droplets under the microscope, I notice that the images appear distorted (for example: the droplets look stretched, compressed, or irregularly shaped).

My questions:

1. What could be the possible causes of image distortion in microfluidic droplet imaging?
2. How can I troubleshoot or correct this distortion?
3. Are there any recommended settings or calibration steps for imaging microfluidic droplets accurately?

I have attached an example image showing the distortion. The image was taken using a 10x objective lens.
Thank you very much for your help!

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