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A desperate newbie can't get the camera to work
Hi all, a newbie here, I yet have very little experience with raspberry pi and electronics in general. Context: I REALLY want to get openflexure to work in order to take images of soil samples. I have...
View Article500 deque mutated during iteration error
I’m encountering a “500 deque mutated during iteration” error while using the OpenFlexure Microscope software. This issue appears when attempting to use the live preview or other functionalities....
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Recurring Low-Cost Camera Problems
We are two college students building the low-cost microscrope, and we keep running into the same few problems. We are unsure if they are related, and would appreciate any help: We keep getting a...
View ArticleSpinning Z axis Gear
I’ve built the Version 7 microscope. The basic electronics work and I get illumination and an image on the screen, but I have a couple of problems. 1) I used cap headed bolts for the large gears and...
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4th motor integration on Sangaboard v0.5
Hi, First of all, thanks for this great device and community! I would like to implement structured illumination on an OFM v7. For this, I want to intergrate a fourth motor (28BYJ-48) on a Sangaboard...
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Dotstar grid illumination issues!
Hello! I’m very happy to report that I’ve printed my delta stage microscope, have assembled it along with assembling an adafruit dotstar LED 8x8. I’ve followed the instructions as per the “LED grid...
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Why is there distortion in my microfluidic droplet images?
Hi everyone, I am currently working on microfluidic droplet generation experiments. When I capture images of the droplets under the microscope, I notice that the images appear distorted (for example:...
View ArticleWorking with sangaboard hardware/library
hey guys, hope you’re all doing well. I’ve been tasked with making the project compatible with a raspberry 5 and so far it has been rough due to my lack of understanding of the inner workings of the...
View ArticleOptics module choice
Can someone explain to me what the reflection optics module does? I keep seeing epi-fluorescence being associated with it but are they equivalent? For example if i want to see the surface of a...
View ArticleSlide positioning
Is the openflexure designed so that the slides are upside down (coverslip downward)? If yes how do you keep the coverslip from falling? Some samples that im interested in are blood, hair, onion skin...
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My lens is too high
Hello dear openFlexure community, I’ve just finished assembling my microscope. However, I’m facing an issue where my objective lens is sticking out too far at the top, and I’m wondering what I might...
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Stripped thread
The thread for my sample clip on the delta stage is too loose. I’m not sure if it was a print defect or me stripping it by accident. Is there anything I could do to fix it except spending another...
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Need help compiling STL
I wanna modify the optics module but I don’t know scad. I’m hoping that someone can help me compile an stl of the optics module for reflection delta stage without the mounting part (I think it’s the...
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Delta_stage openscad structure missing
Hello, I’m very happy and excited to have discovered this impressive project. I am currently using the source scad files of Delta Stage from gitlab and hoping to learn to do some custom design....
View ArticleConcerning serial port commands
Hello, it’s me again, I’m currently trying to make the project work on Raspberry5 with an AI HAT module (hailo-8L) and so far it’s working great, but I still have an issue. I’m using the serial port...
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Delta stage stacking
I can’t seem to make focus stacking work with delta stage using imjoy. Here’s an example of onion skin using all algorithms provided in imagej. I suspect it is due to the Delta stage not being...
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Error in Callibration
Please, how do I fix this? Also, although the motors moved, the stage and objective did not move 2 posts - 2 participants Read full topic
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First-time User staging problems
I have two main issues that I am hoping to get help with. For some background, I purchased the OpenFlexure Microscope through the ioRodeo vendor, and used all of their default equipment, settings,...
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Camera to Stage Mapping problems on upright microscope
Hi, I’m currently using v3 version of server and have both upright and low cost versions of Openflexure microscope. In upright version after calibrating both camera and stage “click to move” function...
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