Measuring Bioluminescence with OpenFlexure v7
Is it possible to measure Bioluminescence with the pi2 camera? I have successfully done this with an iphone because they have some tools that take 30 photos and then merge them all together. But I...
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Objective too big?
I’ve just finished my first build of the microscope (v7). However the objective is about 3mm above the top of the stage, have I bought an objective that’s too long?. Total length is just below 50mm,...
View ArticleBlack Screen When Using OpenFlexure Microscope Camera Need Help!
Hi everyone, Iam having an issue with my OpenFlexure Microscope where the camera feed just shows a black screen instead of displaying the sample. The microscope seems to be connected properly, and the...
View ArticleOpenFlexure Software Loads White Screen – How to Fix?
Hi everyone, Iam encountering a problem where the OpenFlexure Microscope software opens but only displays a white screen with no controls or interface elements. The software doesn’t crash, but I can’t...
View ArticleStepper motors buzzing but not turning with sangaboard v5.5
Hi, I have built a microscope out of curiosity and out of the standard parts listed on the website. I decided to buy he sangaboard v0.5.5 from the preferred supplier. I cannot, however get the...
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Focus distance incorrect
Hi, I am having trouble with the optical assembly on the high resolution microscope. The print was reasonable successful and I have bought the correct spec lens on AliExpress (50mm focal length,...
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Lens question - flatfield correction 4x lens / issues with 1x 2x lens
Hi, When running the auto calibration on my microscope, flatfield correction with the 4x lens does not work as expected, a pink circle remains in the corners of the image: I was able to get rid of the...
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Help with translating the server and a mass distribution project
Hi folks, We at GSIO Georgia, have just finished building the low cost basic optics module v6 microscope and have been testing it extensively with great results ( see pictures below). We are a small...
View ArticleIs my old hardware good enough?
Sorry, I don’t know where to start after seeing your booth at FOSDEM. I couldn’t find a definitive list of recommended parts. I wonder if my old camera v2.1 module here is good to start with...
View ArticleMethods for Determining Minimum Resolution
Hi, given that larger resolution images need more processing power to work with and take up more storage space, it is preferable to have lower resolution images if possible. I was wondering if anybody...
View ArticleWhat is the vertical travel of the lever per step?
Hi! So my esp32-based controller box for the Delta Stage is almost 100% complete. I have added end stop switches into the feet and they work really well. However, since the project is for 2P...
View ArticleDelta stage accumulated error
Hello, first of all thanks for this amazing project. I’m impressed by what has been achieved here. I have just finished putting together a delta stage microscope using the latest version of...
View ArticleNo open serial port found on delta stage with Sangaboard V0.2
Hi everyone, I have built an OpenFlexure Delta Stage microscope with a Sangaboard V0.2. For the Sangaboard V0.2, I ordered the PCB online and then soldered the circuit components onto the PCB myself....
View ArticleIntermittent Black Screen on OpenFlexure Live View Seeking Troubleshooting Tips
Hi Everyone, Iam experiencing an intermittent black screen issue in the live view of my OpenFlexure microscope. The video feed occasionally goes completely blank, even though the microscope itself...
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Leg struts fail to print
Experienced 3D printer here, first time trying to print the OpenFlexure on our Ultimaker S5 FDM 3D Printer using PLA. As I hope you can see from the attached image, the horizontal struts that span the...
View ArticleHow to home the motors?
Hello, there is something I do not quite understand abou the OpenFlexure. ■ How are the motors homed?, and ■ How to proceed if, e.g. while first installing and getting familiar with it, the focus is...
View ArticleInitial focus/calibrate problems after build completion
I have a number of issues post-build trying to focus on my first test slide. I’m listing them in case they are related to each other. I cannot get the slide to focus. What I see on the screen is...
View ArticleI've snapped twenty o-rings
I just snapped my twentieth o-ring trying to install them. I’ve been successful ~4 times. I’m using the recommended o-rings straight from McMaster-Carr. I put a lot of force on the large gear, pushing...
View ArticleBuilding a microscope for meteorite viewing
Hi, I’m interested in building either the normal microscope or the delta stage to put in a museum (behind glass) and ‘fly’ over a meteorite sample. Later ideas might include letting the visitors...
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A desperate newbie can't get the camera to work
Hi all, a newbie here, I yet have very little experience with raspberry pi and electronics in general. Context: I REALLY want to get openflexure to work in order to take images of soil samples. I have...
View Article500 deque mutated during iteration error
I’m encountering a “500 deque mutated during iteration” error while using the OpenFlexure Microscope software. This issue appears when attempting to use the live preview or other functionalities....
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Recurring Low-Cost Camera Problems
We are two college students building the low-cost microscrope, and we keep running into the same few problems. We are unsure if they are related, and would appreciate any help: We keep getting a...
View ArticleSpinning Z axis Gear
I’ve built the Version 7 microscope. The basic electronics work and I get illumination and an image on the screen, but I have a couple of problems. 1) I used cap headed bolts for the large gears and...
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4th motor integration on Sangaboard v0.5
Hi, First of all, thanks for this great device and community! I would like to implement structured illumination on an OFM v7. For this, I want to intergrate a fourth motor (28BYJ-48) on a Sangaboard...
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Dotstar grid illumination issues!
Hello! I’m very happy to report that I’ve printed my delta stage microscope, have assembled it along with assembling an adafruit dotstar LED 8x8. I’ve followed the instructions as per the “LED grid...
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