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A white stain on the camera image
Hi! I finished building the delta stage reflection microscope, and I received an image of the sample but with a white stain: I changed the cameras and Raspberry Pi board and still have this issue, so...
View ArticleAsk for fluorescence light source suggestion
I am currently using a 20W LED light source, but even with an exposure time of 99998, I still cannot capture the fluorescent dye. I have also used a 3W LED with equal intensity across various...
View ArticleWill buy a complete design of a fluorescent microscope
Willing to purchase a working design of a fluorescent microscope with 20X and 40X magnification, motorized scan (0.5mm steps), LED/Laser illumination, interchangeable filters, autofocus, 20Mp camera....
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Is Scan Speed Normal or Low?
Hi! When performing a 10x10 grid scan with a z-step of 1, the process took 53 minutes and generated 900 images, each with size about 600k. Upon switching to debug log mode, I observed that each stage...
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Stage movement too shaky and slide scan help
Hi, Just finished building motorised microscope with low-cost optics. I used 0.8:1 ratio gears for speed. Also since I didn’t have sangaboard and illumination pcb, I used alternatives, and used white...
View ArticleControlling Microscope not available
Hey, it’s me again with probably another stupid question. I am not sure where I am going wrong here. Basically I am looking to find the program to use the Microscope interface as seen here: Use your...
View ArticleAccessing the Raspberry Pi Camera Openflexure Connect video stream
How would I go about accessing this video stream to send to a streaming service such as YouTube (or maybe transfer over MQTT). From what I understand only one app can access the Raspberry Pi Camera at...
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Sangaboard problem
Hello everyone, I need your help. My Sangaboard suddenly can’t connect to the Raspberry Pi, and it keeps showing the error: "ERROR: 'MissingStage' object has no attribute 'board'". What should I do to...
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Illumination kit X effect
I have recently upgraded my microscope with the new illumination kit. But in some areas, I am getting this strange “X” effect. Anyone experiences something like this? 4 posts - 4 participants Read...
View ArticleOFM delta stage calibration failed
Dear all, I constructed the OFM delta using an RPI4. During the process of calibrating the stage, it consistently experiences failures. I am inexperienced in construction. May I inquire if there are...
View ArticleOpenFlexure for Hela Cells in Mammalian CO2 Incubator
Dear OpenFlexure Community, I am working on a project where I need to get a constant visual feed on the evolution of Hela cells placed inside a co2 incubator. It looks like the OpenFlexure could...
View ArticleAutomating the Microscope
Hey there, it’s me again! As I have previously mentioned in another post, I am kinda new to all this coding, whatsoever. What I am planing will probably sound simple to most of you guys, yet it’s...
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Raspberry Pi 5 support
So I have assembled the entire OpenFlexure high-res microscope and included the SD card with the custom OS image in the raspberry pi 5 4GB. Unfortunately, there does not seem support for the Raspberry...
View ArticleUsing step/direction controllers
Has anyone modified sangaboard software to drive 2-pin step/direction controllers (like DRV8825)? 3 posts - 3 participants Read full topic
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Structural relevance of tiny bridges
Hi. I accidentally broke 2 small bridges of one actuator in the main body of the delta stage. Is this a problem or are they of low functional importance? (i.e. should i reprint or not?) The bridges in...
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I finally built my microscope! But the optics doesn't work
Hi! I recently decided to assemble and try the openflexure. The build was smooth and easy, everything worked and I was happy to see something on my screen and the illumination working! I bought some...
View ArticleX axis motor won't stop
Hi! Just finished building the microscope and it works really great. However, there is one issue with controlling the stepper motors: The X axis motor often won’t stop turning (and continues even...
View ArticleFast z-stack recording
Is there a way to perform a fast z-stack (similar to autofocus, so no stops in between) but retrieve the images and relative z-steps? 9 posts - 4 participants Read full topic
View ArticleSuggestions on livestreaming the camera feed to YouTube
@kenzoat worked a bit on this, and I believe when he tried to follow normal setup instructions for accessing the camera, as might be expected it was already in use by the microscope app. It wasn’t...
View ArticleTube lens for infinity corrected objective
Hi, I am currently building a delta stage microscope and decided to use a infinity corrected objective. According to the assembly guide I should get a achromatic tube lens with f = 50mm for the 160mm...
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